Tandem Mass Spectrometry with Ultrahigh Mass Accuracy Clarifies Peptide Identification by Database Retrieval
نویسندگان
چکیده
منابع مشابه
Peptide identification by tandem mass spectrometry with alternate fragmentation modes.
The high-throughput nature of proteomics mass spectrometry is enabled by a productive combination of data acquisition protocols and the computational tools used to interpret the resulting spectra. One of the key components in mainstream protocols is the generation of tandem mass (MS/MS) spectra by peptide fragmentation using collision induced dissociation, the approach currently used in the lar...
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Bioinformatics is a hybrid science at the interface between biology and computer science. For nearly two centuries, data acquisition in the wet lab and in the field has limited the pace of progress in biology. With the advent of high-throughput technologies such as genome sequencing, data analysis has become an additional bottleneck, and simultaneously a rich source of computational and statist...
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In high-throughput proteomics the development of computational methods and novel experimental strategies often rely on each other. In certain areas, mass spectrometry methods for data acquisition are ahead of computational methods to interpret the resulting tandem mass spectra. Particularly, although there are numerous situations in which a mixture tandem mass spectrum can contain fragment ions...
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2007 I To my wife Bai Hong, mother and father You all deserve the pride! II Acknowledgements I would like to first thank my family, especially my parents and my wife, for their endless support every day, month and year during my pursuit of PhD. I would like to take this opportunity to thank Prof. Leong Hon Wai for his patience, constant guidance and countless insightful suggestions throughout m...
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The identification of peptides from tandem mass spectra is an important part of many high-throughput proteomics pipelines. In the high-throughput setting, the spectra are typically identified using software that matches tandem mass spectra with putative peptides from amino-acid sequence databases. The effectiveness of these search engines depends heavily on the completeness of the amino-acid se...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2008
ISSN: 1535-3893,1535-3907
DOI: 10.1021/pr800635m